Can activators enhance potentially developmentalcapacity of 2-cell blocked mouse embryos?

Effects of Visible Light on the Development of Mouse 2-Cell Embryos

Introduction: Mammalian embryos as well as oocytes are prone to various doses of visible light during manipulations in laboratory. The present study was designed to investigate the effects of visible light on the development of mouse 2- cell embryos. Method: Non-pregnant female NMRI mice were super-ovulated with i.p. injection of 7.5 iu PMSG followed 48 hours later with 10 iu hCG. Forty eight h...

Blastomere Fusion of Mouse 2-cell Embryos by Electric Stimulus

The present study was undertaken to find out the suitable methods for blastomere fusion of mouse 2-cell embryos using electric stimulus. Two-cell mouse embryos were treated with electric stimuli under different conditions, and those that showed blastomere fusion were cultured for 3 days to observe their subsequent development. The results obtained are as follows. 1). The proportion of embryos f...

Efficacious cryopreservation of 2 - cell stage mouse embryos via

S Introduction ince Whittingham et al. first succeeded in cryopreserving mouse embryos in 1972, there has been remarkable progress in both basic and applied research enabling us to preserve the embryos of at least 13 more mammalian species. Moreover, every process used for freezing and thawing embryos has been refined and extremely simplified. Specifically: 1) The time required for the embryo t...

effect of recombinant human leukemia inhibitory factoron development of 2-cell mouse embryos in vitro

introduction: to improve the development of embryo in vitro several attempts have been made, including the application of leukemia inhibitory factor (lif). this factor plays an important role in embryo development and has been shown to enhance in vitro development rate in sheep and mice. there is controversy regarding the effect of lif on the different stages of embryo development. the aim of t...

The Effects of Ethanol and Strontium on Growth and Development of Two-Cell Arrested Mouse Embryos