Callus Production in Geranium (Pelargonium quercetorum Agnew) Growing Naturally in Türkiye

Pelargonium quercetorum Agnew grows naturally in the Hakkari province of Türkiye. Although P. has potential use as a medicine and ornamental plant, it is especially used medicinal plant for cure various diseases by local people. In vitro tissue culture methods are favorable propagation, conservation, breeding plants. We aimed this study to achieve regeneration from different explant types. Seeds were germinated conditions explants taken these sterile plantlets. Totally four experiments, containing three them embryogenic one organogenic culture, established Agnew. Leaf, petiole, cotyledon, cotyledon stalk, root collar disc explant. Different concentrations 1-Naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic (2,4-D), 6-Benzylaminopurine (BA), 6-Furfurylaminopurine (Kinetin), 6-(γ,γ-Dimethylallylamino) purine (2iP), Thidiazuron (TDZ) induce or regeneration. Explants cultured half-strength full-strength Murashige Skoog (MS) medium. callus formation media ranged 63.5% 100%, types 39% 100%. experiment, 12.5% 71% 93%. Also, embryo-like structures obtained experiments. However, could not grow more transformed into