Callus and cell suspension culture of bamboo plant, Phyllostachys nigra

Regeneration of Stevia Plant Through Callus Culture

Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to main...

Growth of Rice Root-derived Callus Tissue in Suspension Culture.

Callus Induction and Cell Suspension Culture Production of Catharanthus Roseus for Biotransformation Studies of (–)- Caryophyllene Oxide

For biotransformational studies, Callus of Catharanthus roseus was produced from young shoot tip on Murashige & Skoog medium supplemented with 1.5 mg/litre 2, 4-D and 0.5 mg/litre Kin, solidified by 8 g/litre agar at 25±1°C under complete darkness. The cultures were maintained on the same medium at 25±1 °C in the dark by subculturing after every four weeks. Suspension culture of the plant was p...

Epigenomic Consequences of Immortalized Plant Cell Suspension Culture

Plant cells grown in culture exhibit genetic and epigenetic instability. Using a combination of chromatin immunoprecipitation and DNA methylation profiling on tiling microarrays, we have mapped the location and abundance of histone and DNA modifications in a continuously proliferating, dedifferentiated cell suspension culture of Arabidopsis. We have found that euchromatin becomes hypermethylate...

Establishment of callus and cell suspension cultures of Corydalis saxicola Bunting, a rare medicinal plant.

An efficient procedure has been developed for callus induction and cell suspension cultures of C. saxicola for the first time. Explant selection was carried out among leaf, stem and root to select a suitable type of explants capable of higher callus formation. Leaf explants thus selected showed maximum response to callus induction (67.1%). Modified B5 medium supplemented with 0.5 mg l(-1) 2,4-D...