Calls for New Reporting Standards, Quality Control in Microarrays
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چکیده
منابع مشابه
Calls for new reporting standards, quality control in microarrays.
C oncerns about the quality of microarray data in three Duke University cancer trials have simmered for several years. Then in July, after evidence surfaced regarding alleged inconsistencies in reporting of credentials by one of the Duke researchers, the issue came to a boil: More than 30 bioinformatics and biostatistics specialists sent a letter to National Cancer Institute director Harold Var...
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AIMS To improve the interpretation of immunohistochemistry (IHC) staining results the use of a tissue microarray technique was established in a routine setting. METHODS A tissue microarray was constructed by harvesting 600 microm tissue cores from paraffin wax embedded samples available in a routine pathology department. The punches originating from non-tumorous tissue were placed on host par...
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We present a framework for detecting probes in oligonucleotide microarrays that may add significant error to measurements in hybridization experiments. Four types of so-called degenerate probe behavior are considered: secondary structure formation, self-dimerization, cross-hybridization, and dimerization. The framework uses a well-established model for computing the free energy of nucleic acid ...
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ژورنال
عنوان ژورنال: JNCI Journal of the National Cancer Institute
سال: 2010
ISSN: 0027-8874,1460-2105
DOI: 10.1093/jnci/djq373