Callose synthase and xyloglucan endotransglucosylase gene expression over time in Citrus × clementina and Citrus × sinensis infected with citrus tristeza virus

Abstract Citrus tristeza virus (CTV) is a that already caused great losses in citrus producing regions. The cell wall of plant cells plays an important role the defence response to viruses. Following several studies indicating enzyme transcripts callose synthase 7 ( c alS7 ) and xyloglucan endotransglucosylase 9 xth9 are modified during viral infection, transcript expression calS7 isoform x5 x 5 was evaluated over time × sinensis ‘Valencia Late’ (VL) clementina ‘Fina’ (CL), infected with severe CTV isolate T318A, by quantitative (q) PCR. qPCR analysis healthy performed at 15 days, 10 months 31 post-inoculation (dpi/mpi), respectively. titer, three time-points qPCR, increased bark tissues, VL plants exhibiting titer about times higher than CL mpi. infection did not cause significant changes gene both cultivars. However, associated up-regulation compared controls This study highlights can affect specific wall-associated genes this influence distinct for CL. provides further insight into CTV-citrus host interaction, long-term involving high gene.