Calixarene capture of partially unfolded cytochrome c
نویسندگان
چکیده
منابع مشابه
Rate of Intrachain Diffusion of Unfolded Cytochrome c
How fast can a protein fold? The rate of initial collapse from the unfolded state to a compact structure provides one upper limit to the folding rate. Although hydrophobic collapse of heteropolymers is not well understood, its rate may be controlled by the rate at which contacts form between distant parts of an unfolded polypeptide chain. The rate of this intrachain diffusion has not been measu...
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How fast can a protein fold? The rate of polypeptide collapse to a compact state sets an upper limit to the rate of folding. Collapse may in turn be limited by the rate of intrachain diffusion. To address this question, we have determined the rate at which two regions of an unfolded protein are brought into contact by diffusion. Our nanosecond-resolved spectroscopy shows that under strongly den...
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Unfolding turns immobilized cytochrome c into a His-His ligated form endowed with catalytic activity towards O(2), which is absent in the native protein. Dioxygen could be used by naturally occurring unfolded cytochrome c as a substrate for the production of partially reduced oxygen species (PROS) contributing to the cell oxidative stress.
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Proteins are denatured in aqueous urea solution. The nature of the molecular driving forces has received substantial attention in the past, whereas the question how urea acts at different phases of unfolding is not yet well understood at the atomic level. In particular, it is unclear whether urea actively attacks folded proteins or instead stabilizes unfolded conformations. Here we investigated...
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A zymogen-derived protein, pepsin, appears to be incapable of folding to the native state without the presence of the prosegment. To better understand the nature of the irreversible denaturation of pepsin, the present study reports on the characterization of the stability and low-resolution tertiary and secondary structures of native, alkaline unfolded and acid refolded porcine pepsin. Through ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2019
ISSN: 0014-5793,1873-3468
DOI: 10.1002/1873-3468.13512