Calcium in quinoprotein methanol dehydrogenase can be replaced by strontium.
نویسندگان
چکیده
منابع مشابه
In silico studies of the mechanism of methanol oxidation by quinoprotein methanol dehydrogenase.
The mechanism of bacterial methanol dehydrogenase involves hydride equivalent transfer from substrate to the ortho-quinone PQQ to provide a C5-reduced intermediate that subsequently rearranges to the hydroquinone PQQH(2). We have studied the PQQ reduction by molecular dynamic (MD) simulations in aqueous solution. Among the five simulated structures, either Asp297 or Glu171 or both are ionized. ...
متن کاملReplacement of enzyme-bound calcium with strontium alters the kinetic properties of methanol dehydrogenase.
Methanol dehydrogenase (MEDH) possesses tightly bound Ca2+ in addition to its pyrroloquinoline quinone (PQQ) prosthetic group. Ca2+ was replaced with Sr2+ by growing the host bacterium, Paracoccus denitrificans, in media in which Ca2+ was replaced with Sr2+. MEDH, which was purified from these cells (Sr-MEDH), exhibited an increased absorption coefficient for the PQQ chromophore, and displayed ...
متن کاملCan Anaphoric Definite Descriptions be Replaced by Pronouns?
Abstract We investigate the hypothesis that a pronoun is used in discourse, when its antecedent is in the focus of the discourse. We create a corpus of ‘replaced anaphoric definite descriptions’, where occurrences of definite descriptions are replaced with a corresponding pronoun. We use the Lappin and Leass (1994) anaphora resolution algorithm on the new corpus, and obtain a much lower perform...
متن کاملCatalytic mechanism of quinoprotein methanol dehydrogenase: A theoretical and x-ray crystallographic investigation.
The catalytic mechanism of the reductive half reaction of the quinoprotein methanol dehydrogenase (MDH) is believed to proceed either through a hemiketal intermediate or by direct transfer of a hydride ion from the substrate methyl group to the cofactor, pyrroloquinoline quinone (PQQ). A crystal structure of the enzyme-substrate complex of a similar quinoprotein, glucose dehydrogenase, has rece...
متن کاملThe structure of the quinoprotein alcohol dehydrogenase of Acetobacter aceti modelled on that of methanol dehydrogenase from Methylobacterium extorquens.
The 1.94 A structure of methanol dehydrogenase has been used to provide a model structure for part of a membrane quinohaemoprotein alcohol dehydrogenase. The basic superbarrel structure and the active-site region are retained, indicating essentially similar mechanisms of action, but there are considerable differences in the external loops, particularly those involved in formation of the shallow...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.54.2833