Calcium and methyl jasmonate cross-talk in the secondary metabolism of grape cells

In grape cell cultures cv. Gamay Fréaux var. Teinturier, Ca was shown to decrease pigmentation through the inhibition of anthocyanin biosynthesis, while stimulating stilbenoids accumulation. Because methyl jasmonate (MeJA) is a well-known inducer secondary metabolism in cells, and antagonizes its stimulatory effect over several enzymes core metabolic branches, present study we hypothesized that MeJA signaling pathways interact regulate specific routes. Grape cultured cells were elicited with or + an UPLC-MS-based targeted metabolomic method implemented characterize their polyphenolic profiles. Results compared profile only, previously reported. Data complemented gene expression analysis, allowing assembly map unraveled routes specifically regulated by both elicitors. boosted E-resveratrol E-ε-viniferin levels 180% 140%, respectively, comparison treated flavonoid synthesis inhibited Ca. parallel, downregulated most pathway genes, including LAR1, ANS, BAN ANR. able mimic potentiate on JA JAR1, PIN PR10. Transcript/metabolite correlation networks exposed central influence FLS1, STS, CDPK17 COI1 biosynthetic This highlights potential MeJA-Ca combination for diverting towards production metabolites interest, highly relevant biotechnological perspective.