CaCl2–HCl electrolyzed water promotes glucosinolate metabolism in broccoli sprouts via calcium signalling

Abstract Background Electrolyzed water, a green chemistry invention, is novel disinfectant that has been widely used in the food field. Previous studies have found slightly acidic electrolyzed water not only bactericidal effect but also promotes accumulation of active substances many types sprouts. We developed new type CaCl 2 –HCl (CHEW), which can effectively enhance formation glucosinolates and isothiocyanates broccoli The molecular mechanism by glucosinolate metabolism sprouts during growth was investigated this study. Results On 4th day, total content reached 195.50 μmol/g DW under CHEW treatment. 8th compared to treatment with tap contents three (sulforaphane, erucin, 4-isothiocyanato-1-butene) were significantly increased 45.93, 34.04 185.15%, respectively. results calcium distribution qRT‒PCR assessments indicated activated signalling promoted relative expression biosynthesis-related genes through sensors [calmodulin (CaM), calmodulin-like protein (CML), calcium-dependent kinase (CDPK)] calcium-binding nuclear IQD1. Conclusion Our research provides insights into theoretical basis for cultivating high-quality Graphical