Bypass of heterology during strand transfer by Saccharomyces cerevisiae Rad51 protein
نویسندگان
چکیده
منابع مشابه
Bypass of heterology during strand transfer by Saccharomyces cerevisiae Rad51 protein.
During recombination-mediated repair of DNA double-strand breaks, strand transfer proteins must distinguish a homologous repair template from closely related genomic sequences. However, some tolerance by strand transfer proteins for sequence differences is also critical: too much stringency will prevent recombination between different alleles of the same gene, but too much tolerance will lead t...
متن کاملThe DNA binding properties of Saccharomyces cerevisiae Rad51 protein.
Saccharomyces cerevisiae Rad51 protein is the paradigm for eukaryotic ATP-dependent DNA strand exchange proteins. To explain some of the unique characteristics of DNA strand exchange promoted by Rad51 protein, when compared with its prokaryotic homologue the Escherichia coli RecA protein, we analyzed the DNA binding properties of the Rad51 protein. Rad51 protein binds both single-stranded DNA (...
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In this study, solid state fermentation (SSF) was carried out to produce single cell protein (SCP) from sugarcane bagasse using Saccharomyces cerevisiae. The SSF experiment were performed in a tray bioreactor. The influence of several parameters including extraction buffer, initial moisture content of substrate, fermentation time, relative humidity in bioreactor, the bioreactor temperature and ...
متن کاملSaccharomyces cerevisiae Dmc1 and Rad51 preferentially function with Tid1 and Rad54, respectively, to promote DNA strand invasion during genetic recombination
Background: DNA strand exchange proteins, Dmc1 and Rad51, and translocases, Tid1 and Rad54, function in DNA break repair during meiosis. Results: We biochemically demonstrate that Dmc1 and Rad51 are specifically stimulated by Tid1 and Rad54, respectively. Conclusions: Dmc1-Tid1 and Rad51-Rad54 represent functional pairs for DNA pairing and joint molecule formation. Significance: The separate an...
متن کاملThe human Rad51 protein: polarity of strand transfer and stimulation by hRP-A.
The human Rad51 protein is homologous to the RecA protein and catalyses homologous pairing and strand transfer reactions in vitro. Using single-stranded circular and homologous linear duplex DNA, we show that hRad51 forms stable joint molecules by transfer of the 5' end of the complementary strand of the linear duplex to the ssDNA. The polarity of strand transfer is therefore 3' to 5', defined ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2001
ISSN: 1362-4962
DOI: 10.1093/nar/29.24.5052