Bulk Fusion Studies of Sendai Virus and Application of Mass Action Kinetic Fusion Model
نویسندگان
چکیده
منابع مشابه
Studies of membrane fusion. V. Fusion of erythrocytes with non-haemolytic Sendai virus.
The fusion of human erythrocytes with non-haemolytic '1-day' Sendai virus has been studied by electron microscopy. The mechanism of viral envelope-cell fusion is the same as that described previously for haemolytic '3-day' Sendai virus except that fusion is frequently arrested at an initial stage when 2 segments of smooth linear viral membrane fuse and become incorporated into the erythrocyte m...
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We have studied the fusion activity of Sendai virus, a lipid-enveloped paramyxovirus, towards a line of adherent cells designated PC-12. Fusion was monitored by the dequenching of octadecyl-rhodamine, a fluorescent non-exchangeable probe. The results were analysed with a mass action kinetic model which could explain and predict the kinetics of virus-cell fusion. When the temperature was lowered...
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1. to determine whether difference in birth body mass influenced growth performance in pipistrellus kuhlii we studied a total of 12 captive-born neonates. bats were assigned to two body mass groups: light birth body mass (lbw: 0.89 ± 0.05, n=8) and heavy birth body mass (hbw: 1.35 ± 0.08, n=4). heavier body mass at birth was associated with rapid postnatal growth (body mass and forearm length) ...
Viral-induced fusion of human cells. I. Quantitative studies on the fusion of human diploid fibroblasts induced by Sendai virus.
Human diploid fibroblasts in monolayer cultures underwent cytoplasmic fusion in the presence of UV-inactivated Sendai virus. The proportion of nuclei in the cultures which were in polykaryocytes (“polykaryocytic index”) under the conditions used in the present study, was about 0.50. When the cells were fused by the methods developed for the present study, the polykaryocytic index was substantia...
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The effect of divalent cations on cell fusion by concentrated Sendai virus, inactivated by beta-propiolactone, was investigated using Vero and mouse L-929 cells in monolayers. With both cell lines, which are normally resistant to exogenous viral fusion, Cu(2+) in sublethal concentrations was found to promote polykaryon formation to a marked degree. The simultaneous presence of Cu(2+) and virus ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2021
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2020.11.2025