Broadening of analyte streams due to a transverse pressure gradient in free-flow isoelectric focusing
نویسندگان
چکیده
منابع مشابه
Microfluidic Protein Separation by Free Flow Isoelectric Focusing
Disposable, inexpensive microfluidic devices have the potential to become a robust tool for proteomic research for separating and identifying proteins and protein complexes. In this work, a preparative scale free flow IEF isoelectric focusing (FFIEF) device was designed, investigated, and optimized. A constant electric field of up to 350V/cm was generated across the separation channel upon opti...
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We demonstrate the fabrication, characterization and application of microfluidic chips capable of continuous electrophoretic separation via free flow isoelectric focussing (FFIEF). By integration of a near-infrared (NIR) fluorescent pH sensor layer under the whole separation bed, on-line observation of the pH gradient and determination of biomolecular isoelectric points (pI) was achieved within...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2017
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2017.01.004