Brilliant Cresyl Blue Staining Enhances the Morphological Quality Evaluation of Porcine Oocytes-a Preliminary Study

Abstract Brilliant cresyl blue (BCB) staining is a method used for selection of developmentally competent porcine oocytes, in addition to the standard morphological classification. The aim current study was assess relationship between BCB oocytes with high and low characteristics its maturation rate. In first part study, cumulus-oocytes complexes (COCs) (n=434) were aspirated from 60 ovaries. According classification staining, they divided 4 groups: COC+/BCB+, COC−/BCB+, COC+/BCB−, COC−/BCB−. second experiment, COCs (n=445) categorized into 3 control, BCB+, BCB−. Significant differences rate observed COC+/BCB+ COC−/BCB− (66% vs. 23%), COC−/BCB+ 38%) (p<0.0001). also registered COC−/BCB-(38% 23%) COC+/BCB− (53% (p<0.01). Results experiment showed that only BCB− had significantly lower (28%) compared control (63%) BCB+ (59%) (p<0.001). These findings higher developmental ability morphology. BCB-staining high-quality did not have significant impact on their group, but it might be useful selecting morphology characteristics.