Bright labeling with MoonTag

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Bright and stable CdSe/CdS@SiO₂ nanoparticles suitable for long-term cell labeling.

We report on the synthesis of luminescent CdSe/CdS@SiO2 nanoparticles and their application to cell labeling. The main novelty of these nanoparticles is the use of newly developed "flash" CdSe/CdS quantum dots (QDs), which are obtained through a new fast and efficient synthesis method recently reported. These core-shell QDs are encapsulated in silica nanoparticles through a water-in-oil microem...

متن کامل

Bright sinus appearance on arterial spin labeling MR imaging aids to identify cerebral venous thrombosis

Cerebral venous thrombosis is a potentially lethal disease. Early diagnosis is essential to improve its prognosis. However, its early diagnosis based on conventional imaging modalities remains a challenge in clinical settings. The purpose of this study was to evaluate whether bright sinus appearance on arterial spin-labeling perfusion-weighted image (ASL-PWI) could help identify cerebral venous...

متن کامل

Solving the riddle of the bright mismatches: labeling and effective binding in oligonucleotide arrays.

RNA binding to high-density oligonucleotide arrays has shown tantalizing differences with solution experiments. We analyze here its sequence specificity, fitting binding affinities to sequence composition in large datasets. Our results suggest that the fluorescent labels interfere with binding, causing a catch-22. To be detected, the RNA must both glow and bind: without labels it cannot be seen...

متن کامل

mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures

A monomeric variant of the red fluorescent protein eqFP611, mRuby, is described. With excitation and emission maxima at 558 nm and 605 nm, respectively, and a large Stokes shift of 47 nm, mRuby appears particularly useful for imaging applications. The protein shows an exceptional resistance to denaturation at pH extremes. Moreover, mRuby is about ten-fold brighter compared to EGFP when being ta...

متن کامل

RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking.

Because RNA lacks strong intrinsic fluorescence, it has proven challenging to track RNA molecules in real time. To address this problem and to allow the purification of fluorescently tagged RNA complexes, we have selected a high affinity RNA aptamer called RNA Mango. This aptamer binds a series of thiazole orange (fluorophore) derivatives with nanomolar affinity, while increasing fluorophore fl...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Nature Methods

سال: 2019

ISSN: 1548-7091,1548-7105

DOI: 10.1038/s41592-019-0517-5