منابع مشابه
A phosphoprotein phosphatase from ox brain.
1. The pyrophosphate-exchange reactions which are catalysed by rat-liver preparations and depend upon leucine or isoleucine are profoundly modified by 'soluble' ribonucleic acid and by changes in magnesium concentration. The preponderant influence is exerted by the terminal nucleotide sequence of the 'soluble' ribonucleic acid. Lysinedependent pyrophosphate exchange occurs only at relatively hi...
متن کاملBrain dolichyl pyrophosphate phosphatase. Solubilization, characterization, and differentiation from dolichyl monophosphate phosphatase activity.
Dolichyl [beta-32P]pyrophosphate ([beta-32P]Dol-P-P) has been prepared chemically to study Dol-P-P phosphatase in calf brain. Calf brain microsomes catalyze the enzymatic release of 32Pi from exogenous [beta-32P]Dol-P-P by a bacitracin-sensitive reaction. [32P]Pyrophosphate was not detected with the water-soluble product even when 1 mM sodium pyrophosphate was added to impede pyrophosphatase ac...
متن کاملProperties of pure acyl phosphatase from bovine brain.
Acyl phosphatase has been purified 25,000-fold from bovine brain. The enzyme is more than 97% pure by acrylamide gel disc electrophoresis. The calculated molecular weight from gel filtration measurements on Sephadex G-75 was 12,100; the value calculated from the amino acid composition was 8,732. Independent determinations of free -SH groups and tyrosine content were in agreement with the amino ...
متن کاملDifferential expression of protein phosphatase 1 isoforms in mammalian brain.
Rat cDNAs encoding neuronal isoforms of protein phosphatase 1 (PP1) were isolated and their primary structures elucidated. The derived amino acid sequences allowed us to design synthetic C-terminal peptides that were used to raise antibodies. Isoform-specific anti-peptide antibodies against PP1 alpha and PP1 gamma 1 were used to investigate the tissue distribution of PP1 isoforms by immunoblott...
متن کاملModulation of calcium-activated potassium channels from rat brain by protein kinase A and phosphatase 2A.
By incorporating plasma membrane vesicles into planar lipid bilayers, we previously characterized a family of four types of Ca(2+)-activated K+ channels from rat brain (Reinhart et al., 1989). Two of these are "large-conductance" or "maxi"-K+ channels, which differ in their gating kinetics and toxin sensitivity and are henceforth referred to as "type 1" and "type 2" channels. Here we show that ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1936
ISSN: 0306-3283
DOI: 10.1042/bj0301089