<b>PRIMARY MONOLAYER CULTURE OF ADULT RAT HEPATOCYTES IN A SERUM-FREE SYSTEM: METABOLIC AND MORPHOLOGICAL </b><b>STUDIES </b>
نویسندگان
چکیده
منابع مشابه
Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiations
Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbecco's medium supplemented with 17% fetal calf serum. The ultrastructure of monolayer cells was examined after various incubation periods. Within 4 h of plating, the isolated spherical cells adhere to the plastic surface, establish their first contacts by numerous i...
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Adult rat hepatocytes cultured on type IV collagen, fibronectin, or laminin and maintained in serum-free medium were examined by indirect immunofluorescence using polyclonal antibodies against extracellular matrix proteins. An extensive fibrillar matrix containing fibronectin and fibrin was detected in all hepatocyte cultures irrespective of the exogenous matrix substratum used to support cell ...
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Hepatic parenchymal cells from adult rats, established in vitro as a monolayer, have been evaluated by electron microscopy. Within 24 h after the initial seeding, the incubated cells were polygonal and in close apposition with three to six neighboring cells. The ultrastructure of the monolayer cells was examined at this time and after 3 and 10 days of incubation. With the exception of a few enl...
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stimulated cells: 2.63.5 nmol/l O6 cells). The 0;production was dependent on the presence of oxidative substrates such as glucose and succinate. Mast cells, on the other hand, did not release 0;upon activation with IgE antibody or compound 48/80, although both the stimuli caused substantial release of histamine and serotonin. However, both mast cells and macrophages Benerated significant amount...
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We have studied the effect of 3,5,3'-triiodothyronine (T3) on the respiration of adult rat hepatocytes in primary monolayer culture prepared from hypothyroid rat liver. After addition of T3 to the culture medium at a concentration of 2 x 10(-7) M, oxygen consumption of the cultured cells increased detectably at 24 h and was maximal at 72--96 h, relative to control cultures (38.0 +/- 1.8 vs. 25....
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ژورنال
عنوان ژورنال: Biomedical Research
سال: 1981
ISSN: 0388-6107,1880-313X
DOI: 10.2220/biomedres.2.491