Bovine Oocytes Can Be Penetrated in Modified Tris-buffered Medium
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چکیده
منابع مشابه
Bovine Oocytes Can Be Penetrated in Modified Tris-buffered Medium
A modified Tris-buffered medium (mTBM) has been widely used as an insemination medium for porcine in vitro fertilization (IVF). We examined whether mTBM could be used for bovine IVF. Bovine cumulus-oocyte complexes (COCs) were cultured in a serum-free medium containing 30 ng/ml EGF for 22 h. After culture, COCs were inseminated with spermatozoa for 12 h in mTBM containing 5 mM caffeine and 10 g...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2009
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.2009.80431