Bovine eggs release zinc in response to parthenogenetic and sperm-induced egg activation
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چکیده
منابع مشابه
Sperm Chromatin-Induced Ectopic Polar Body Extrusion in Mouse Eggs after ICSI and Delayed Egg Activation
Meiotic chromosomes in an oocyte are not only a maternal genome carrier but also provide a positional signal to induce cortical polarization and define asymmetric meiotic division of the oocyte, resulting in polar body extrusion and haploidization of the maternal genome. The meiotic chromosomes play dual function in determination of meiosis: 1) organizing a bipolar spindle formation and 2) indu...
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Parthenogenetic activation of Lytechinuspictus eggs can be monitored after inje&ion with the Ca-sensitive photoprotein aequorin to estimate calcium release during activation. Parthenogenetic treatments, including the nonelectrolyte urea, hypertonic sea water, and ionophore A23187, all acted to release Ca2+ from intracellular stores. Ionophore and urea solutions release Ca*’ from the same intrac...
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Bull spermatozoa were pellet frozen on dry ice in a TES buffer containing combinations of egg yolk and glycerol to determine which component of an extender afforded cryoprotection to the spermatozoa. Neither buffer nor buffer plus glycerol protected sperm cell motility in the absence of egg yolk, while sperm cells frozen in egg yolk buffer in the absence of glycerol had 24% postthaw motility (P...
متن کاملSoluble sperm factors and Ca2+ release in eggs at fertilization.
All eggs are activated at fertilization by an increase in intracellular free Ca2+. How the spermatozoa triggers the release of intracellular Ca2+ has not been established in any species. One hypothesis is that spermatozoa introduce a Ca(2+)-releasing factor into the cytoplasm after gamete membrane fusion. It has been suggested that spermatozoa may introduce Ca2+ itself, or a small molecular wei...
متن کاملEfficient Method For Combined Electrical-Chemical Parthenogenetic Activation of Bovine Oocytes
Purpose: Parthenogenetic activation is among the crucial steps determining successful development of mammalian cloned embryos. This study, therefore, was conducted to evaluate the efficiency of a novel combined electrical-chemical artificial activation method for bovine cloning. Materials and Methods: In vitro matured bovine oocytes than were initially exposed to electrical pulse, used for cel...
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ژورنال
عنوان ژورنال: Theriogenology
سال: 2019
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2018.12.031