Bone Tissue Engineering Scaffold Optimisation through Modification of Chitosan/Ceramic Composition

A large bone defect is defined as a that exceeds the regenerative capacity of bone. Nowadays, autologous grafting still gold standard treatment. In this study, hybrid tissue engineering scaffold (BTE) was designed with biocompatibility, biodegradability and adequate mechanical strength primary objectives. Chitosan (CS) biocompatible biodegradable polymer can be used in wide range applications engineering. Hydroxyapatite (HAp) fluorapatite (FAp) have potential to improve properties CS. present work, different volumes acetic acid (AA) ratios HAp FAp scaffolds were prepared UV cross-linked form 3D structure. The characterised by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, swelling studies compression testing. cytotoxicity result obtained MTT assay. degradation rate tested weight loss after immersed SBF. results showed crosslinked structure formed bonding occurred between materials within scaffold. Additionally, not only provided sufficient but also cytocompatibility, depending on their composition. degraded gradually 6-to-8-week testing period, which closely matches regeneration rates, indicating BTE field.