BN nanospheres as CpG ODN carriers for activation of toll-like receptor 9
نویسندگان
چکیده
منابع مشابه
Cutting edge: Role of Toll-like receptor 9 in CpG DNA-induced activation of human cells.
Unmethylated CpG motifs present in bacterial DNA stimulate a rapid and robust innate immune response. Human cell lines and PBMC that recognize CpG DNA express membrane-bound human Toll-like receptor 9 (hTLR9). Cells that are not responsive to CpG DNA become responsive when transfected with hTLR9. Expression of hTLR9 dramatically increases uptake of CpG (but not control) DNA into endocytic vesic...
متن کاملToll-like receptor 9-dependent immune activation by unmethylated CpG motifs in Aspergillus fumigatus DNA.
Phagocytic defenses are critical for effective host defenses against the opportunistic fungal pathogen Aspergillus fumigatus. Previous studies found that following challenge with A. fumigatus, Toll-like receptor 9 (TLR9) knockout mice survived longer than wild-type mice. However, the mechanism responsible was not defined. Here we demonstrate that A. fumigatus contains unmethylated CpG sequences...
متن کاملNuclease-resistant immunostimulatory phosphodiester CpG oligodeoxynucleotides as human Toll-like receptor 9 agonists
BACKGROUND Unmethylated cytosine-guanine (CpG) motif-containing oligodeoxynucleotides (ODNs) have been well characterized as agonists of Toll-like receptor 9 (TLR9). ODNs with a phosphorothioate (PTO) backbone have been studied as TLR9 agonists since natural ODNs with a phosphodiester (PD) backbone are easily degraded by a serum nuclease, which makes them problematic for therapeutic application...
متن کاملinteraction of cpg-oligodeoxynucleotides with toll like receptor 9 induces apoptosis and modulates metaloproteinase-2 activity in human intestinal epithelium
recent reports have indicated different effects of immunostimulatory sequences containing cpg-oligodeoxynucleotides (odn) on various immune cells. however, the exact role of cpg-odn in the human gut is unclear. in the present study, we assessed potential effects of cpg-odn on non lymphoid cell (intestinal epithelial cell line ht-29) on a dose-response and time-course basis. intestinal epithelia...
متن کاملPreparation and characterization of PLGA nanospheres loaded with inactivated influenza virus, CpG-ODN and Quillaja saponin
Objective(s): The purpose of this study was preparation and evaluation of PLGA nanospheres containing the influenza virus and different adjuvants, Quillaja saponin (QS) and CpG-ODN. Materials and Methods: Nanospheres were prepared using the double emulsion-solvent evaporation method. The morphological and physicochemical properties were studied by scanning electron microscopy...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Materials Chemistry
سال: 2011
ISSN: 0959-9428,1364-5501
DOI: 10.1039/c1jm10199d