منابع مشابه
Chromosome end protection by blunt-ended telomeres.
Single-stranded telomeric DNA protrusions are considered to be evolutionarily conserved structural elements essential for chromosome end protection. Their formation at telomeres replicated by the leading strand mechanism is thought to involve poorly understood post-replicative processing of blunt ends. Unexpectedly, we found that angiosperm plants contain blunt-ended and short (1- to 3-nucleoti...
متن کاملEnd protection
www.sciencemag.org (this information is current as of December 14, 2008 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/320/5881/1344 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/1158441/DC1 can be found a...
متن کاملBlunt-end Cloning of PCR Products.
INTRODUCTION Incubation of a blunt-end ligation reaction in the presence of an excess amount of an appropriate restriction enzyme can dramatically increase the yield of recombinant plasmids. The role of the restriction enzyme is to cleave circular and linear concatemers at restriction sites that are re-formed when linear, blunt-ended plasmid molecules ligate to themselves. In almost all cases, ...
متن کاملPlasmids with Blunt - End Restriction Enzyme 1
TA cloning is one of the simplest and most efficient methods for the cloning of PCR products. The procedure exploits the terminal transferase activity of certain thermophilic DNA polymerases, including Thermus aquaticus (Taq) polymerase. Taq polymerase has non-template dependent activity which preferentially adds a single adenosine to the 3'-ends of a double stranded DNA molecule, and thus most...
متن کاملA-tailing Protocol for Blunt-end Fragments
Pfu DNA Polymerase(a)-generated blunt-end PCR fragments can be ligated into the pGEM®-T and pGEM®-T Easy Vectors(b,c) if the fragments are first A-tailed using Taq DNA Polymerase(a). We describe a method for A-tailing these PCR fragments and demonstrate its utility by cloning two different Pfu DNA Polymerase-generated fragments into the pGEM®-T Easy Vector, using the new 2X Rapid Ligation Buffe...
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ژورنال
عنوان ژورنال: Science
سال: 2012
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.337.6096.778-d