Blue fluorescent protein from the calcium-sensitive photoprotein aequorin is a heat resistant enzyme, catalyzing the oxidation of coelenterazine
نویسندگان
چکیده
منابع مشابه
Peroxidized coelenterazine, the active group in the photoprotein aequorin.
The photoprotein aequorin emits light by an intramolecular reaction when Ca2+ is added under either aerobic or anaerobic conditions. Previously reported evidence has indicated two possibilities: (i) the functional group of aequorin is coelenterazine itself, a compond that plays key roles in the bioluminescence of various other types of organisms, or (ii) it is the enolized form of this compound...
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The luminescent protein aequorin from the jellyfish Aequoria victoria emits light by an intramolecular reaction in the presence of a trace amount of Ca(2+). In order to understand the mechanism of the reaction, a study of structure-function relationships was undertaken with respect to modifying certain of its amino acid residues. This was done by carrying out oligonucleotide-directed site-speci...
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Calcium signaling and Aequorin Aequorin is a photoprotein originating from the jellyfish Aequorea Victoria (Inouye et al., Proc. Nat. Acad. Sci. USA 82, 3154-3158, 1985; Prasher et al., Biochem. Biophys. Res. Commun. 126 :1259-1268, 1985). The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin, the active form ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2004
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2004.09.078