Block of cleavage of ultraviolet-irradiated DNA with restriction endonucleases.
نویسندگان
چکیده
منابع مشابه
Block of cleavage of ultraviolet-irradiated DNA with restriction endonucleases.
pBR322/Ultraviolet irradiation/Restriction endonuclease/Pyrimidine dimer Plasmid pBR322 DNA was irradiated with ultraviolet light at 254 nm and digested with various restric tion endonucleases. Analyses of the products suggested that the pyrimidine dimers which would have been formed just across the cutting site (Acc I), at the opposite position of the cutting site (Hind III, Eco RI, Bam HI), a...
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Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. The first method presented in this unit is the cleavage of a single DNA sample with a single restriction endonuclease. A...
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We have determined the mode of cleavage of superhelical SV40 DNA (Form I) by restriction endonucleases EcoRI and HpaII at 37 degrees C. By analysis with agarose gel electrophoresis and direct examination with dark field electron microscopy, we found that a large amount of the single-nicked circular DNA (Form II) was produced before the linear SV40 DNA (Form III) appeared. Thus, both restriction...
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ژورنال
عنوان ژورنال: Journal of Radiation Research
سال: 1986
ISSN: 0449-3060,1349-9157
DOI: 10.1269/jrr.27.247