Bleomycin resistance: a new dominant selectable marker for plant cell transformation
نویسندگان
چکیده
منابع مشابه
evaluation of spirulina platensis resistance to different antibiotics to find a selectable marker for genetic transformation
background spirulina is one of the most profitable known microalgae in the world, which is used as food and superfood. in the other hand, spirulina is a useful source of healthy components. it seems that the spirulina is transformable so that the introduction of a selectable marker is needed. objectives the purpose of this study was to determine a suitable selection marker for spirulina platens...
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The antibiotic G418 was shown to be an effective inhibitor of vaccinia virus replication when an appropriate concentration of it was added to cell monolayers 48 h before infection. Genetic engineering techniques were used in concert with DNA transfection protocols to construct vaccinia virus recombinants containing the neomycin resistance gene (neo) from transposon Tn5. These recombinants conta...
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Chlorimuronethyl resistance gene is increasingly used as a selectable marker for transformation, especially fungal transformation. Magnaporthe grisea is an important model organism for investigating fungal pathogenicity, and Agrobacterium tumefaciens-mediated transformation (ATMT) is used for functional mutagenesis of the fungus. However, our results showed that rice blast strains collected fro...
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Most strains of Helicobacter pylori are naturally competent for uptake of chromosomal DNA. Transformation frequencies for streptomycin resistance or rifampicin resistance markers ranged from 1 x 10(-4) to 1 x 10(-3) per viable cell using a plate transformation procedure. Transformation of a metronidazole resistance marker (MtrR) was demonstrated when either a laboratory-derived mutant or a MtrR...
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Plastid transformation is widely used in basic research and for biotechnological applications. Initially developed in Chlamydomonas and tobacco, it is now feasible in a broad range of species. Selection of transgenic lines where all copies of the polyploid plastid genome are transformed requires efficient markers. A number of traits have been used for selection such as photoautotrophy, resistan...
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ژورنال
عنوان ژورنال: Plant Molecular Biology
سال: 1986
ISSN: 0167-4412,1573-5028
DOI: 10.1007/bf00021328