Blebs and Apoptotic Bodies Are B Cell Autoantigens

Autoantigens Blebs and Apoptotic Bodies Are B Cell

Blebs and apoptotic bodies are B cell autoantigens.

Mounting evidence suggests that systemic lupus erythematosus autoantigens are derived from apoptotic cells. To characterize the potential interactions between apoptotic cells and B cells, the D56R/S76R variant of 3H9, a murine autoantibody that binds to DNA, chromatin, and anionic phospholipids, was compared with DNA4/1, a human anti-DNA autoantibody. Flow cytometry revealed that only D56R/S76R...

Apoptotic fractions as cell-associated antigen sources: ACR vs blebs

7 CHAPTER 7 W ith an increasing number of clinical successes in targeting both solid and leukemic tumors by tumor infiltrating lymphocytes (TILs) or T cells engineered to express chimeric antigen receptors (CARs), our increased knowledge on DC biology, and the recent remarkable breakthroughs with checkpoint blockade, the field of tumor immunotherapy is clearly advancing. With these advances and...

Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes

Systemic lupus erythematosus is a multisystem autoimmune disease in which the autoantibody response targets a variety of autoantigens of diverse subcellular location. We show here that these autoantigens are clustered in two distinct populations of blebs at the surface of apoptotic cells. The population of smaller blebs contains fragmented endoplasmic reticulum (ER) and ribosomes, as well as th...

P-6: Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies

Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...