Birth of Normal Mice Following Round Spermatid Injection Without Artificial Oocyte Activation
نویسندگان
چکیده
منابع مشابه
Round spermatid injection.
This finding has caused great concern with round spermatid enthusiasts. A major complaint is that “one should not restrict the label of round spermatid only to those cells in which an acrosomal vesicle is visible” (p. 2977) (4). However, our observation is based not only on phase-contrast evaluation of TESE microdroplets but also on detailed stained histology of many hundreds of testis specimen...
متن کاملThe activity (calcium oscillator?) responsible for human oocyte activation after injection with round spermatids is associated with spermatid nuclei.
OBJECTIVE To compare the oocyte-activating ability of whole human round spermatids and their isolated nuclei. DESIGN Prospective study using sibling oocytes from patients undergoing spermatid conception and intracytoplasmic sperm injection treatment cycles. SETTING Private assisted reproduction laboratories and a university department. PATIENT(S) Couples with male infertility. INTERVENT...
متن کاملP-56: Artificial Oocyte Activation Alone Intra Cytoplasmic Sperm Injection in Men with Severe Semen Parameters
Background Failed fertilization occurs 1-3% in the infertile couples. Failure of oocyte activation (OA) is mainly attributed to failed fertilization. ICSI alone artificial oocyte activation (AOA) is only treatment approach for these individuals. Three procedures are introduced for AOA: chemical, mechanical and electrical. Most of the clinical Centers have commonly used ionomycin for activation ...
متن کاملFourteen babies born after round spermatid injection into human oocytes.
During the human in vitro fertilization procedure in the assisted reproductive technology, intracytoplasmic sperm injection is routinely used to inject a spermatozoon or a less mature elongating spermatid into the oocyte. In some infertile men, round spermatids (haploid male germ cells that have completed meiosis) are the most mature cells visible during testicular biopsy. The microsurgical inj...
متن کاملMethods for isolation and purification of post-ejaculate human round spermatids for possible use in intracytoplasmic round spermatid injection*
Objective: To develop methods for the isolation and purification of round spermatids (RS) from ejaculated semen specimens recovered from patients suffering from nonobstructive azoospermia for the purpose of performing intracytoplasmic round spermatid injections (ROSI) into human oocytes. Design: Prospective study. Setting: Audrology Institute of Lexington, Lexington, Kentucky. Patients: Six men...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2011
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.11-008m