Biotransformation of Silver Nanoparticles into Oro-Gastrointestinal Tract by Integrated In Vitro Testing Assay: Generation of Exposure-Dependent Physical Descriptors for Nanomaterial Grouping

Grouping approaches of nanomaterials have the potential to facilitate high throughput and cost effective nanomaterial screening. However, an grouping hinges on application suitable physicochemical descriptors identify similarities. To address problem, we developed integrated testing approach coupling acellular cellular phases, study full life cycle ingested silver nanoparticles (NPs) salts in oro-gastrointestinal (OGI) tract including their impact uptake integrity. This enables derivation exposure-dependent physical (EDPDs) upon biotransformation undigested nanoparticles, digested salts. These are identified in: size, crystallinity, chemistry core material, dissolution, low molecular weight Ag-biomolecule soluble complexes, compared terms similarities a hypothesis. Experimental results indicate that neither similar pristine nor completely salts, due presence different chemical nanoforms (silver chloride nanocrystals), which were characterized interactions with digestive matrices. Interestingly, responses observed phase assay (uptake inflammation) also for samples, clearly indicating possible role fraction complexes. highlights importance quantifying exposure-related advance NPs based structural