Biotransformation of quercetin, kaempferol and apigenin to monoglycosylated derivatives by in vitro suspension cultures of Astragalus vesicarius ssp. carniolicus

Biotransformation of exogenous substrates quercetin, kaempferol and apigenin by suspension cultures Astragalus vesicarius ssp. carniolicus to their monoglycosylated derivatives was performed. The maximal enzymatic potential cells A. evaluated different concentrations substrate exposure. According quantitative ultra-high performance liquid chromatography-high resolution electrospray ionization mass spectrometry (UHPLC-HR-ESI-MS) analysis, the highest concentration O -glycoside (14.88 nmol/g dry weight, DW), (10.55 DW) quercetin (150.83 achieved, when were treated with 4 mg/mL kaempferol, 3 respectively. glycosidic products biotransformation not detected in untreated control.