BIOTIN AND BACTERIAL GROWTH

Bacterial degradation of biotin. Catabolism of 14C-biotin and its sulfoxides.

A biotin-degrading particulate system has been obtained by sonic rupture of a soil pseudomonad which grows on d-biotin as sole source of carbon, nitrogen, and sulfur. Investigation of the catabolism of l*C-biotin and its sulfoxides in this system has revealed the following. Degradation of biotin to CO2 is enhanced in the bufferwashed particulate preparations by addition of adenosine triphosphat...

In vivo biotinylation of bacterial magnetic particles by a truncated form of Escherichia coli biotin ligase and biotin acceptor peptide.

Escherichia coli biotin ligase can attach biotin molecules to a lysine residue of biotin acceptor peptide (BAP), and biotinylation of particular BAP-fused proteins in cells was carried out by coexpression of E. coli biotin ligase (in vivo biotinylation). This in vivo biotinylation technology has been applied for protein purification, analysis of protein localization, and protein-protein interac...

Peptides and Bacterial Growth

Lactobacillus casei grows in the absence of vitamin Ba, provided that D-alanine and an unidentified peptide are added to a medium complete in other nutrients (1, 2). A partial hydrolysate of protein contained several chromatographically distinct fractions that possessed this “peptide factor” activity (3). It appeared likely that a single amino acid linked in a variety of combinations was respon...

Bacterial Growth and Division

Structure and Complexation with Biotin and Biotin-Lipid Assemblies

Fourier-transform infrared studies have been carried out to investigate the secondary structure and thermal stability of hen egg white avidin and its complexes with biotin and with a biotinylated lipid derivative, N-biotinyl dimyristoyl phosphatidylethanolamine (DMBPE) in aqueous dispersion. Analysis of the amide I stretching band of avidin yielded a secondary structural content composed of app...