Biosynthesis of acridone alkaloids formation of rutacridone by cell-free extracts of Ruta graveolens cell suspension cultures
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منابع مشابه
Biosynthesis of Acridone Alkaloids. A Cell-Free System from Ruta graveolens Cell Suspension Cultures
Ruta graveolens, Suspension Cultures, Cell-Free System, Acridone Biosynthesis, 1,3-DihydroxyN-methylacridone A cell-free extract of Ruta graveolens cell suspension cultures, strain R-20, was prepared which synthesizes 1,3-dihydroxy-N-methylacridone from N-methylanthranilic acid and malonylcoenzyme A. The reaction is strongly dependent on the presence of ATP. After adding of coenzyme A to the as...
متن کاملPurification and properties of acridone synthase from cell suspension cultures of Ruta graveolens L.
Acridone synthase has been purified from cell suspension cultures of Ruta graveolens using a combination of gel filtration and ion exchange chromatography. The purified enzyme has an apparent molecular weight of 69 kDa on gel filtration and a subunit structure on SDS-PAGE of 40 kDa. The apparent Km-values are 10.64 microM and 32.8 microM for N-methylanthraniloyl-CoA and malonyl-CoA, respectivel...
متن کامل[Acridine-alkaloids from callus cultures of Ruta graveolens L].
Rutacridone, 1-hydroxy-A-methylacridone, and l-hydroxy-3-methoxy-A-methylacridone have been isolated for the first time from callus cultures, which had been obtained from meristematic stem cells of Ruta graveolens L. The alkaloids were identified by co-chromatography with authentic compounds, by IR spectra, and by electronic spectra before and after addition of A1C13 . The use of the bathochrom...
متن کاملCharacterization of Volatile Constituents from Heterotrophic Cell Suspension Cultures of Ruta graveolens
Heterotrophic cell suspension cultures of Ruta graveolens were established by reversion of photom ixotrophic cultures without any change in the chemical composition of the growth medium. The heterotrophic cultures were qualitatively and quantitatively analyzed by gaschromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, C6-C8 ketones,...
متن کاملFormation of 1,3-Dihydroxy-N-methylacridone from N-Methylanthraniloyl-CoA and Malonyl-CoA by Cell-Free Extracts of Ruta graveolens
Cell-free extracts of Ruta graveolens cell sus pension cultures catalyze the condensation of N-methylanthranilic acid and malonyl-CoA in the presence of ATP and Mg2+ [1,2]. After addition of CoA-SH to the incubation mixture an inhibitory effect on alkaloid formation was observed as was previously described for the chalcone synthase reaction [3, 4]. It may be assumed that the incorpo ration of...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1990
ISSN: 0014-5793
DOI: 10.1016/0014-5793(90)81395-5