Biosurfactants synthesized by endophytic Bacillus strains as control of Moniliophthora perniciosa and Moniliophthora roreri

Ecuador is responsible for up to 70 % of the world’s production fine flavor cocoa. Although fungal diseases have reduced cocoa production, eco–friendly alternatives been implemented control spread phytopathogens. This study aimed explore potential endophytic strains isolated from Nacional trees synthetize compounds with antimicrobial and surface active properties. It was determined that under in vitro conditions, seven (DS03, DS07, DS18, DS23, DS31, DS34 DS50) exhibited antifungal activity inhibited mycelial growth Moniliophthora roreri perniciosa. The were identified based on their morphological (pattern forming Bacilli) genetic characteristics using 16S SSU rRNA gene sequences. Furthermore, we contained genes encoded lipopeptides Fengycin (fenA, fenB, fenC, fenE), Iturin (ituB, ituC, ituD), Bacyllomicin D (bamC, bamD). biosurfactant by a reduction tension mineral medium. Additionally, crude extract (BCE) tested its inhibitory against M. perniciosa roreri. minimal concentration (MIC) be 0.07 0.035 mg mL−1, respectively, both cases fungistatic action mode. swelling granulation fragmentation phytopathogens’ hyphae observed BCE treatment. These results suggested biocontrol capacity strains, which ability synthesize might used as an ecological alternative plants prevent infection