Biomolecular Features of COVID-19 in Hodeidah, Yemen

Background: The biomolecular technique namely Real-Time Polymerase Chain Reaction (RT-PCR) is very important in confirmation of coronavirus disease 2019 (COVID-19) but progressive infection and predication the illness severity not used.
 Objective: Therefore, study aimed to determine relationship between viral load COVID-19 based on cycle threshold (Ct).
 Methodology: research was designed a case series study. included 60 patients that were confirmed by RT-PCR test with divided into two major groups. first group mild moderate cases (n:20) treated at home (outpatient) second severe critical (n:40) isolation center (inpatient).
 Results: results showed no illness, where minimum Ct 14, maximum 36. mean 22 ± 7. On other hand, low (high load) reported early detection cases. In addition , 60% (36 cases) had ; ≤ 15 25 ) 40% (24 high (low 26 39 more than less cycles . 95 % (mild -values 42.5 (severe critical) -values. kinetic load–based week as 21) longitudinal assessment individuals requiring third –fourth weeks clear RNA significant reduction samples (Ct values > 30). 
 Conclusion: concluded several factors can affect (onset date, collection technique, type swab, sampling method). Briefly, cannot be used predictor illness.