Biological control of citrus canker pathogen Xanthomonas citri subsp. citri using Rangpur lime endophytic bacteria

Abstract Background Rangpur lime (RL) is a common rootstock cultivar used worldwide. However, it known to have high susceptibility citrus canker (CC). To meet the increasing demand for healthy seedlings, this research aimed study potency of RL endophytic bacteria as biocontrol agent CC pathogen Xanthomonas citri subsp . citri. The isolates were collected from leaves and subjected in vitro planta antagonistic tests against XCC, alongside its cell-free supernatant (CFS). Potential identified according their 16S rDNA sequence similarities. Results As many 21 obtained trees. Two (B1 C8) demonstrated promising inhibitory activity XCC. Based on assays, cell suspensions (CS) these could effectively inhibit growth with an optimum clear zone diameter 8.41 7.51 mm, respectively. Consistent CS, CFSs also displayed similar potencies XCC highest recorded being 7.23 mm 6.22 Further investigations revealed that minimum concentration B1 C8 was 25 µg/ml. It found both sensitive heat very low pH, but stable presence proteinase-K. In vivo assay, CS CFS treatments protect CC. Treated plants had significantly lower disease incidences developed less severe symptoms than control plants. sequence, Staphylococcus pasteuri warneri , Conclusion concluded be agents These bacterial can alternative prevention measure opposed commonly chemicals.