Biolistic-mediated Transformation of Lilium longiflorum cv. Nellie White
نویسندگان
چکیده
منابع مشابه
Biolistic gun-mediated maize genetic transformation.
Biolistic gun-mediated transformation is one of the two most effective and popular methods for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature e...
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Lily species (Lilium spp.) are highly appreciated for their aesthetic value as cut flowers or pot plants and have been among the most important ornamental species worldwide for a long time. Numerous lily breeding programs aim at the establishment of novel phenotypes with higher market values. Until now these programs are based mainly on segregation and selection of natural variants and interspe...
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Lilium species (Liliaceae) are a significant floriculture commodity and one of the three major bulb crops in the commercial market. Lilies are monocotyledonous plants that have been generally recalcitrant to molecular genetic manipulation because of limitations that restrict utilization of transformation technologies that are routinely applied to dicotyledonous plants. Cucumber mosaic virus (CM...
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Although various animal tissues are used for bioassay of aflatoxins (B1, B2, G1, G2), a rapid bioassay dependent upon a plant part's response does not exist. Both pollen germination (G) and tube elongation (TE) were enhanced in a 3.0 mM KH2PO4 (K)-containing but AFB1-lacking, modified Dickinson's medium. The B1 did not affect G when K was withheld but K supplementation impaired G above 15 micro...
متن کاملA Calcium-Activated Phytase from Pollen of Lilium longiflorum.
A phytase was isolated and partially purified from the pollen of Lilium longiflorum Thumb. Optimum activity was at pH 8.0. The phytase was activated by Ca(2+) and Sr(2+) but not by the other divalent cations tested. Activity was inhibited by ethylenediaminetetraacetate. The phytase had a temperature optimum of 55 to 60 degrees C and an activation energy of about 12,700 calories/mole. Extraction...
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ژورنال
عنوان ژورنال: HortScience
سال: 2008
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.43.6.1864