Bioleaching of lateritic nickel ore with acidophilic bacteria

PCR-mediated detection of acidophilic, bioleaching-associated bacteria.

The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.

Low-Temperature Carbothermic Reduction of Indonesia Nickel Lateritic Ore with Sub-Bituminous Coal

In this work, the experiment was conducted to investigate the effect of temperature and sub-bituminous coal on upgrading nickel laterites ores by carbothermic reduction. The reduction was carried out in the muffle furnace at a temperature ranging of 800-1100 o C. The produced calcine from reduction step was grounded in vibrating mill, mixed with water and passed on a magnetic separator to separ...

Bioleaching of Low Grade Manganese Ore with Penicillium citrinum

A native microorganism, Penicillium citrinum was isolated from the top soil of a manganese mine. Based on its efficiency for manganese solubilisation, it was utilized for the leaching of a low-grade manganese ore. The effects of various parameters such as pulp density, particle size, sucrose concentration, inoculum size and bioleaching duration on manganese ore were studied. The optimised condi...

Comparative Genomics Unravels the Functional Roles of Co-occurring Acidophilic Bacteria in Bioleaching Heaps

The spatial-temporal distribution of populations in various econiches is thought to be potentially related to individual differences in the utilization of nutrients or other resources, but their functional roles in the microbial communities remain elusive. We compared differentiation in gene repertoire and metabolic profiles, with a focus on the potential functional traits of three commonly rec...

Bioleaching of Copper from Low-Grade Ore Using Isolated Bacteria and Defined Mixed Cultures