Bioimpedance single cell sensing of low and high density sickle erythrocytes using microfluidics

In this paper, sickled red blood cells of different densities from distinct Sickle Cell Disease (SCD) patients were analyzed, using a microfluidic device based on bioimpedance sensing. Complementary to our previous work (Xu et al., 2020) demonstrating the capacity discriminate between normal and pathological cells, we investigated microcirculatory properties (RBCs) in sickle cell disease their density. RBCs separated triple density Percoll fractionation low (LD), dense (D) high (HD) RBCs. Single transiting through constrictions was electrically recorded. Gaussian distributions, adjusted histograms transit time (TT), as well phase blockade amplitude (IP BA) shift (PS) electrical signal, analyzed for three RBC subpopulations. A specific signature achieved each subpopulation, with decreased TT, BA PS increasing densities. This approach provides promising tool monitor effect therapeutical strategies other disorders. • Bioimpedance monitoring single constrictions. Discrimination erythrocytes percoll fractionation. Altered tissue oxygenation impacted by disturbed oxygen delivery due lower contact surface vascular wall. Promising