Bioglass/carbonate apatite/collagen composite scaffold dissolution products promote human osteoblast differentiation

OssiMend® Bioactive (Collagen Matrix Inc., NJ) is a three-component porous composite bone graft device of 45S5 Bioglass/carbonate apatite/collagen. Our in vitro studies showed that conditioned media the dissolution products OssiMend stimulated primary human osteoblasts to form mineralized bone-like nodules one week, basal culture (no osteogenic supplements). Osteoblast differentiation was followed by gene expression analysis and mineralization assay. In contrast, from commercial (Bioglass-free carbonate apatite/collagen scaffolds), or Bioglass particulate alone, did not induce extracellular matrix, but osteoblast mature osteoblasts, evidenced strong upregulation BGLAP IBSP mRNA levels. The calcium ions soluble silicon species released particles additional phosphorus release mediated osteostimulatory effects. Medium with had much higher concentration than alone. While led precipitation cell media, produced OssiMend. These results suggest adding produces synergistic osteostimulation effect on osteoblasts. summary, scaffold (OssiMend® Bioactive) stimulate matrix without any supplements. faster for ordinary Bioglass.