Biogenic Synthesis of Selenium Nanoparticles using Diospyros montana Bark Extract: Characterization, Antioxidant, Antibacterial, and Antiproliferative Activity

Selenium nanomaterials (Nano-Se) are new selenium sources with excellent biocompatibility, degradability, and bioactivities. The objective of the present study is green synthesis nanoparticles (SeNPs) using Diospyros montana Roxb (DM) bark extract, its characterization, evaluation for in-vitro antioxidant, antibacterial anticancer activities. To synthesize montana- (DM-SeNPs), selenious acid (H2SeO3) was reduced D. extract via precipitation technique. UV-Vis, FTIR, XRD, SEM, EDAX, ICP-AES were used to characterize DM-SeNPs. DPPH free radical scavenging assay reducing power capacity test DM-SeNP antioxidant activity. properties tested well diffusion method against gram-positive gram-negative microorganisms. DM-SeNPs also subjected antiproliferative activity MTT MCF-7 cell line. A peak in UV at 289 nm validated According DLS, TEM images, size between 100-150 nm. XRD analysis confirmed crystallinity verified colloidal dispersion EDAX analysis, content 63.45 ±18.3 µg/mL DM-SeNP. IC50 24.72 ± 0.63 EC50 46.30 0.21 values indicated that had a good capacity. showed comparative better potential. inhibition zones found be highest E. coli (48.00 mm), B. subtilis (44.14 Klebsiella pneumonia (36.20 S. aureus (34.16mm), respectively. Antiproliferative carried out, which cytotoxic breast cancer cells line (MCF-7). 38.19 0.27 Doxorubicin 6.41 0.09 µg/mL, suggests display moderate cytotoxicity could dose-dependently inhibit proliferation. Thus, experimental evidence provides insight into nanoparticle synthesis, potential therapeutic value, prospect developing formulation containing