Bioelectroanalytical Detection of Lactic Acid Bacteria
نویسندگان
چکیده
Lactic acid bacteria (LAB) are an industrial important group of organisms that notable for their inability to respire without growth supplements. Recently described bioelectroanalytical detectors can specifically detect and enumerate microorganisms depend on a phenomenon known as extracellular electron transport (EET) effective detection. EET is often type microbial respiration, which logically excludes LAB from such detection platform. However, members the have recently been electroactive with ability carry out EET, providing timely impetus revisit utility in Here, we show LAB, Enterococcus faecalis, easily detected bioelectroanalytically using defined substrate resorufin-β-d-galactopyranoside. Detection rapid, ranging 34 235 min inoculum sizes between 107 104 CFU mL−1, respectively. We that, although signal achieved by faecalis comparable systems rely respiratory strategies target bacteria, E. not dependent electrode energy, it only necessary capture small amounts organism’s metabolic energy to, this case 1.6%, achieve good The results pave way new means detecting industrially organisms, particularly food industry.
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ژورنال
عنوان ژورنال: Applied sciences
سال: 2022
ISSN: ['2076-3417']
DOI: https://doi.org/10.3390/app12031257