Biocontrol potential of Chitosan extracted from Procambarus clarkii (Crustacea: Cambaridae) against Eobania vermiculata snails (Muller 1774) in Egypt
نویسندگان
چکیده
Abstract Background Land snails, especially the chocolate banded Eobania vermiculata (Muller 1774) are destructive pests of a wide range field and vegetable crops, biological treatment appears to be better alternative chemical snail control. Therefore, goal this work was assess molluscicidal activity chitosan extracted from crawfish Procambarus clarkii against E. using oxidative stress, histopathological genotoxic biomarkers. Results Exposure snails LC 50 (222.4 mg/l) for 1, 3 7 days induced significant increase in glutathione S-transferase catalase levels then decline reduced content after 1 as well slight decrease CAT levels, GSH GST treated exposure. Histologically, stress by exposure leads deformation cells, dilatation intertubular spaces, destruction tubules with lumen size, necrosis digestive cells rise vacuoles number calcium number. Considerably, great damage observed increasing time Furthermore, genotoxicity assessed RAPD-PCR technique results revealed that change RAPD profiles following included loss normal DNA bands appearance new one compared control snails. The genomic template stability 63.6, 36.4 18.2% exposure, respectively. apparent increased decreased, while GTS values decreased confirming effect chitosan-induced damage. Conclusion Chitosan may an ecofriendly acceptable pesticide
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ژورنال
عنوان ژورنال: Egyptian Journal of Biological Pest Control
سال: 2022
ISSN: ['2536-9342', '1110-1768']
DOI: https://doi.org/10.1186/s41938-022-00521-x