Biochemical transformation of deoxythymidine kinase-deficient mouse cells with UV-irradiated equine herpesvirus type 1

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Purification and biochemical characterization of deoxythymidine kinase of deoxythymidine kinase-deficient mouse 3T3 cells biochemically transformed by equine herpesvirus type 1.

A line of mouse 3T3 cells lacking deoxythymidine kinase (dTK-) was stably transformed to see dTK+ phenotype after exposure to ultraviolet-irradiated equine herpesvirus type 1 (EHV-1). Deoxythymidine kinase (dTK) was purified from the biochemically transformed mouse cells by affinity chromatography on deoxythymidine-Sepharose. The purified dTK from EHV-1-transformed 3T3 cells was identical to th...

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Equine Herpesvirus Type 1 Enhances Viral Replication in CD172a+ Monocytic Cells upon Adhesion to Endothelial Cells.

UNLABELLED Equine herpesvirus type 1 (EHV-1) is a main cause of respiratory disease, abortion, and encephalomyelopathy in horses. Monocytic cells (CD172a(+)) are the main carrier cells of EHV-1 during primary infection and are proposed to serve as a "Trojan horse" to facilitate the dissemination of EHV-1 to target organs. However, the mechanism by which EHV-1 is transferred from CD172a(+) cells...

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Protein kinase activity in equine herpesvirus.

A protein kinase which is intimately associated with equine herpesvirus (equine abortion virus) was found by using adenosine triphosphate-gamma-(32)P as a phosphate donor and virus protein as an acceptor. Consistent demonstration of the activity requires prior removal of phosphohydrolase. The kinase activity requires Mg(2+), is not stimulated by cyclic adenosine monophosphate, but is enhanced b...

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Title : A retrospective study on equine herpesvirus type - 1 associated

Diagnosis of equine herpesvirus-1 associated myeloencephalopathy (EHM) can be troublesome, but early recognition and knowledge of risk factors are essential for prevention and control. The objectives for this study are to 1) describe EHM in France, 2) improve clinical recognition, 3) identify risk factors. Through epidemiosurveillance of acute neurological cases (all considered to be potentiall...

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Equine herpesvirus-1 consensus statement.

Equine herpesvirus-1 is a highly prevalent and frequently pathogenic infection of equids. The most serious clinical consequences of infection are abortion and equine herpesvirus myeloencephalopathy (EHM). In recent years, there has been an apparent increase in the incidence of EHM in North America, with serious consequences for horses and the horse industry. This consensus statement draws toget...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1978

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.28.1.361-367.1978