Biochemical characterization and intracellular localization of the Menkes disease protein
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چکیده
منابع مشابه
BHK21 myosin: isolation, biochemical characterization and intracellular localization.
Myosin has been isolated from baby hamster kidney cells (BHK21/C13) in high yield and characterized biochemically and immunologically. The subunit composition consists of 2 heavy chains, approximately 200,000 Daltons each, and 2 classes of light chains of approximately 16,000 and 20,000 Daltons. The myosin exhibits ATPase activity in the presence of K+-EDTA or Ca2+ but very little activity with...
متن کاملDefective copper-induced trafficking and localization of the Menkes protein in patients with mild and copper-treated classical Menkes disease.
Menkes disease is an X-linked disorder of copper metabolism. An overall copper deficiency reduces the activity of copper-dependent enzymes accounting for the clinical presentation of affected individuals. The Menkes gene product (MNK) is a P-type ATPase and is considered to be the main copper efflux protein in most cells. The protein is located primarily at the trans -Golgi network (TGN), but r...
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متن کاملA Golgi localization signal identified in the Menkes recombinant protein.
Menkes disease arises from a genetic impairment in copper transport. The gene responsible for the phenotype has been identified as a copper transporting ATPase ( ATP7A ). Recently, the protein encoded by the ATP7A gene has been localized to the Golgi complex. In order to investigate the role of the Menkes disease protein in copper transport, recombinant constructs containing both the full-lengt...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1996
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.93.24.14030