Biochemical and Cell Biological Analyses of a Mammalian Septin Complex, Sept7/9b/11
نویسندگان
چکیده
منابع مشابه
The Mammalian Septin Interactome
Septins are GTP-binding and membrane-interacting proteins with a highly conserved domain structure involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. To date, 13 different septin genes have been identified in mammals (SEPT1 to SEPT12 and SEPT14), which can be classified into four distinct subgroups based on the sequence homology of t...
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Gin4, a Nim1-related kinase, is required in budding yeast for localization of the septins and for proper control of daughter cell growth during G2/M. Gin4 becomes hyperphosphorylated when cells enter mitosis, leading to activation of Gin4 kinase activity. In this study, we have used immunoaffinity chromatography to identify proteins that associate with Gin4 during mitosis, with the goal of find...
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Septins form a filamentous collar at the mother-bud neck in budding yeast. In cytokinesis, this collar splits into two rings and the septin complexes undergo a dramatic reorientation. Using fluorescence polarization microscopy, DeMay et al. (2011. J. Cell Biol. doi:10.1083/jcb.201012143) now demonstrate that septin complexes assemble as paired filaments in vivo and reveal new insights into sept...
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Chartreusin (CT), an antibiotic produced by Strepto myceschartreusls,was recentlyfoundto be activeagainst experimental tumors B16 melanoma and L1210 and P388 leukemia. This report describes the correlation between ftsbiologicalandbiochemicaleffectson mouseL1210and P388leukemIa. CT was growthInhibitoryto L1210cells and other mam malian cells tested (drugconcentrationrequiredfor 50% inhibition of...
متن کامل3D reconstruction of mammalian septin filaments.
Mammalian septins are a family of guanosine triphosphate-binding proteins thought to play a role in a number of key cellular processes, such as cytokinesis, protein scaffolding and vesicle trafficking. Although their precise functions remain to be determined, electron microscopy has shown septin filament formation in vitro and a role as a cytoskeletal polymer has been proposed. Here, we present...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m406153200