Bioaerosol Contribution to Atmospheric Particulate Matter in Indoor University Environments

Within the framework of project “Integrated Evaluation Indoor Particulate Exposure”, we carried out a 4-week field study to determine indoor bioaerosol, and its contribution particulate matter (PM)10 organic matter. The was in university classrooms, where most common sources atmospheric particles are missing. Bioaerosol determined by method based on propidium iodide staining, observation fluorescence microscopy, image analysis. bioaerosol concentrations were compared with outdoor values, which simultaneously. samplings periods scheduled divide weekday hours, when students inside, from night-time hours weekends. Very high detected inside classrooms respect values. mean difference 49 ?g/m3 5.4 during night, it became negative Indoor-to-outdoor ratios 6.0, 4.2, 0.7, respectively. contributed 26% organics 10% PM10. In samples collected day, microscope images showed numerous skin fragments, mostly responsible for increase mass. People’s presence proved be significant concentration crowded environments.