Bioactive Glass Flakes as Innovative Fillers in Chitosan Membranes for Guided Bone Regeneration

Herein, novel bioactive glass flakes (BGFs) (45S5 composition), obtained by compression in the liquid phase a stirred media mill, are combined with chitosan (CS) to develop composite membranes using solvent casting for guided bone regeneration (GBR). The incorporation of BGFs endows CS superior bioactivity as evidenced rapid hydroxyapatite formation after 3 days immersion simulated body fluid (SBF). Moreover, addition reduces swelling ratio membranes, beneficial GBR applications. behavior can also be modulated tuning added amount BGFs. In vitro cell studies indicate noncytotoxicity toward osteoblast line MC3T3-E1, although presence slightly viability compared pure membranes. improves osteogenic differentiation MC3T3-E1 cells indicated enhanced alkaline phosphatase activity. results show feasibility anisotropic fillers polymeric matrices. conclusion, developed CS-BGF promising potential material GBR.