Bioactive component analysis and investigation of antidiabetic effect of Jerusalem thorn (Paliurus spina-christi) fruits in diabetic rats induced by streptozotocin

The extracts of Jerusalem thorn fruits (JT-FE) have been commonly used for the treatment diabetes mellitus in Turkey. In this study, it is aimed to investigate effects JT-FE, prepared by decoction, on blood glucose, insulin and glycated haemoglobin levels diabetic rats induced with streptozotocin (STZ). Hypoglycemic activity was examined streptozotocin-induced rats. For purpose, pre-prandial sugar, hemoglobin were measured. To active substances that responsible antidiabetic activity, phytochemical analysis carried out optimized validated LC-MS/MS method using 53 phytochemicals JT-FE. addition, ICP-OES performed determine mineral content. findings study demonstrate when JT-FE applied groups compared control group, their sugar seen statistically decrease (p < 0,001). Morewer, When treated insulin-treated groups, a 0,05) observed. On other hand, also found increase extract concentration didn't contribute significantly activity. As result analysis, total 31 different phenolic compounds defined major components (as analyte/g extract) were; rutin (98753.4 ± 24.39 ?g), catechin (58695.3 12.971 hesperidin (47445.2 15.894 quinic acid (38279.5 14.239 ?g) malic (17536.8 2.279 ?g). we made; Sodium, calcium, magnesium phosphorus elements at macro level, Zn Cr3+ minerals trace level. Our show rich content strong antihyperglycemic That's why can be useful phytotherapy agent.