Bioacetoin Production by Bacillus subtilis subsp. subtilis Using Enzymatic Hydrolysate of Lignocellulosic Biomass

Acetoin is an important bio-product useful in the chemical, food and pharmaceutical industries. Microbial fermentation major process for production of bioacetoin, as petroleum resources used chemical methods are depleting day by day. Bioacetoin using wild microorganisms easy, eco-friendly economical method bioacetoin. In present study, culture conditions nutritional requirements were optimized bioacetoin a non-pathogenic strain B. subtilis subsp. JJBS250. The bacterial produced maximum (259 mg L−1) peptone (3%) sucrose (2%) at 30 °C, 150 rpm pH 7.0 after 24 h. Further supplementation combinatorial nitrogen sources, i.e., (1%) urea (0.5%), resulted enhanced titre (1017 culture. An approximately 46.22–fold improvement was achieved optimization process. analysis samples thin layer chromatography confirmed presence filtrate. enzymatic hydrolysate obtained saccharification pretreated rice straw sugarcane bagasse cellulase from Myceliophthora thermophila. Fermentation 210 473.17 mgL−1 respectively. Enzymatic hydrolysates supplemented with source showed two to four-fold Results have demonstrated utility type JJBS250 potential making use renewable cost-effective lignocellulosic substrate. Therefore, this study will help sustainable management agricultural waste industrial combating environmental pollution.