Bio-production enhancement of Natural diosgenin from Balanites Aegyptiacae by callus culture

In vitro the production of natural diosgenin from callus culture became an alternative biotechnological approach to production. This study describes effect plant growth regulators (PGRs) on induction and using different elicitors in induce (Diosgenin) parts Balanites Aegyptiacae extract cell lines. Root, stem, leaves calture B. aegyptiacae, were raised Murashing skoog Medium (MS) supplemented with both NAA (Naphthaline Acetic acid) 2,4D (2,4 – Dichlorophenoxy acetic as at concentration. Then Elicitation process was applied by adding amino acids coconut milk concentrations medium. root which 300mg/Ltryptophane gave maximum content spectrophotometric method HPLC analysis. cytotoxic activity tryptophan determined against HepG2 (human hepatocellular carcinoma line), A549 (Human Lung Cancer line) result 51.26 μm/ml, 28.9 μm/ml respectively. The bio safety detected normal human cells 287.3 μm/ml.