Bio-inspired trypsin-chitosan cross-linked enzyme aggregates: a versatile approach for stabilization through carrier-free immobilization
نویسندگان
چکیده
منابع مشابه
Use of Cross-Linked Enzyme Aggregates (CLEAs) for performing biotransformations
The methodology for the preparation of cross-linked enzyme aggregates (CLEAs) and their application as highly effective, recyclable biocatalysts in a wide variety of transformations is reviewed. The method constitutes a combination of enzyme purification and immobilization into a single operation and allows for the use of relatively crude enzyme preparations. The resulting CLEAs have excellent ...
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The compositions and the multi phase structures of bio-nanocomposite hydrogels made from silicate cross-linked PEO and chitosan are related to some of their physical and biological properties. The gels are injectable and self-healing because the cross-linking is physical and reversible under deformation. The presence of chitosan aggregates affects the viscoelastic properties and reinforces the ...
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Stabilization of multimeric enzymes is one of the major challenges in biocatalysis, because dissociation of subunits can inactivate the enzyme. Catalase (CAT; EC 1.11.16) is a homotetramer containing Fe-protoporphyrin IX in its active site. CAT breaks down hydrogen peroxide into water and molecular oxygen. In this study, crosslinked enzyme aggregates of bovine liver CAT (CAT-CLEAs) were prepare...
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The key to obtaining an optimum performance of an enzyme is often a question of devising an effective method for its immobilization. In the present review, we describe a novel, versatile and effective methodology for enzyme immobilization as CLEAs (cross-linked enzyme aggregates). The method is exquisitely simple (involving precipitation of the enzyme from aqueous buffer followed by cross-linki...
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A novel chemiluminescent immunosensor for carbohydrate antigen 19-9 (CA19-9) based on the immobilization of CA19-9 on the cross-linked chitosan membrane was developed. The different membranes were characterized by atomic force microscopy (AFM) and infrared spectrum, respectively. Based on a noncompetitive immunoassay format, this proposed chemiluminescent immunosensor enabled a low-cost, flexib...
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ژورنال
عنوان ژورنال: BioTechnologia
سال: 2019
ISSN: 0860-7796
DOI: 10.5114/bta.2019.87589