Binding of Carbon Monoxide to alpha-Hemocyanin and beta-Hemocyanin from Helix pomatia
نویسندگان
چکیده
منابع مشابه
[Small-angle x-ray-scattering studies on the substructure of Helix pomatia hemocyanin].
Hemocyanin of the snail helix pomatia was studied by small angle X-ray scattering in 0.15 M phosphate buffer pH 6. The outer portion of the scattering curve showed minima, which indicated that the hemocyanin molecule is composed of a large number of identical (or very similar) nearly spherical subunits. From the position of the minima the radius of gyration R u of the subunit was calculated to ...
متن کاملSmall-angle X-ray scattering studies of 1-10-size and 1-20-size molecules of Helix pomatia alpha-hemocyanin.
The small-angle X-ray scattering of 1/10-size molecules (in 0.1 ionic strength Tris buffer pH 8.1) and 1/20-size molecules (in 0.1 ionic strength ethanolamine buffer pH 10.6) of a-hemocyanin of the snail Helix pomatia was studied. The radii of gyration, 8.7 nm and 8.0 nm for 1/10-size and 1/20-size molecules respectively, and the shape of the scattering curves indicate that both have a very ani...
متن کاملLuminescence of carbon monoxide hemocyanins.
The effect of carbon monoxide on the luminescence properties of Helix pomatia alpha-hemocyanin and Panulirus interruptus hemocyanin has been studied. These proteins, when saturated with carbon monoxide, show, besides the intrinsic fluorescence arising from the aromatic amino acid residues, emission in the visible region with a maximum between 540 and 560 nm. Results of carbon monoxide titration...
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The in vivo oxygen-binding characteristics of Cancer magister whole hemolymph were compared across developmental stages with those of purified hemocyanin. When the 25S hemocyanins from first-instar juvenile and adult C. magister were dialyzed against first-instar juvenile saline, the P50 values at pH 7.8 differed by 54 %: 2.16 kPa for the adult and 4.68 kPa for the first-instar juvenile. Since ...
متن کاملSDS-induced phenoloxidase activity of Helix aspersa maxima hemocyanin
Oxygen-transporting protein of the hemolimph of snails Helix aspersa maxima (HaH) was converted from being an oxygen carrier to a form which exhibited phenoloxidase activity by incubation with sodium dodecyl sulfate (SDS). On treatment with 1.73 mM SDS, for 3 min, significant increase of catalytic efficiency of hemocyanin towards substrate catechol (kcat/Km = 34.56 mM.min -1 versus kcat/Km = 0....
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1976
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1976.tb10829.x